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In the presence of guide RNA (gRNA) and Cas9 endonuclease, the gRNA directs the Cas9 endonucleases to cleave the DNA at a specific location, creating indels in the gene, inducing a frameshift mutation, generating a constitutive knockout

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J Cutan Med Surg

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Metabolism and function of coenzyme

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Nucleic Acids Symposium Series 41 , 9598 (1999)

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