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qPCR analysis was performed to measure the mRNA level of c-Fos ( g ), c-Jun ( h ) and Smurf1 ( i ) in indicated cells (n = 4 technical repeats from one of three independent experiments)

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ALI was induced via intratracheal administration of 1 g/g LPS dissolved in 50 l of saline

The SF solution was centrifuged twice at 11,000 RPM to remove insoluble particulates, resulting in a final concentration of 78% (w/v), which was used without dilution

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