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RNA spike-ins were purified using AMPure XP beads (Beckman Coulter), quantified using Qubit HS RNA Assay kit (Invitrogen), and mixed at a final concentration of 1 ng/L of each spike-in RNA

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Garca et al., 2011)

This balanced interplay of upregulated and downregulated proteins, including the proteasome complex, highlights the intricate regulatory networks that underpin neuronal stability, adaptability, and intercellular communication within the model, emphasizing their vital roles in maintaining the overall health and functionality of the nervous system