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3 Current therapeutic Abs for AGA therapy Therapeutic Abs are rarely developed for AGA therapy due to the requirement to be responsiveness to androgens and to primarily exist in the extracellular fluid or cell surface surrounding the hair follicle

Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C

Vitamin D is the sunshine vitamin that helps boost the immune system, reduce depression, decrease allergy symptoms, reduce joint pain, and improve cognitive functions

Loss of appetite and/or weight loss