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In brief, the sections were incubated for 90 min at 25 C in blocking solution (10% horse serum in PBS), and then overnight at 4 C with primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA) or mouse anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA)

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McLaughlin LA, Niazi U, Bibby J, David JP, Vontas J, Hemingway J, Ranson H, Sutcliffe MJ, Paine MJ: Characterization of inhibitors and substrates ofAnopheles gambiaeCYP6Z2.Insect Mol Biol2008, 17(2):125-135

QUANTIFICATION AND STATISTICAL ANALYSIS Unpaired Students t-tests were used to calculate the p value for pairwise comparisons