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For nanomaterials, this is amplified by their dynamic behaviour

HUVEC and HDMEC were maintained in Endothelial Cell Basal Medium (PromoCell) supplemented with 2% FCS, 0.4% endothelial cell growth supplement, 0.1 ng ml 1 recombinant human EGF (rh EGF), 1 ng ml 1 recombinant human basic fibroblast growth factor, 90 g ml 1 heparin and 1 g ml 1 hydrocortisone

These molecular interactions facilitate activation of the Ras/RAF/MEK/ERK pathway, quantifiable through phospho-specific antibody detection and real-time PCR analysis of immediate early gene expression profiles