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Mechanistic investigations often pair 5-Amino-1MQ treatment with transcriptomic and proteomic analyses to map the downstream signaling changes that accompany NNMT inhibition, establishing a fuller picture of the enzyme's regulatory network

Preclinical studies have shown that this peptide fragment can influence pathways associated with lipid metabolism and intracellular signalling without eliciting broad growth hormonemediated effects

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Materials and methods Cell culturing RWPE-1 (ATCC CRL-11609) epithelial cells and DU-145 (ATCC HTB-81) human prostate carcinoma cells were purchased from American Type Culture Collection (Manassas, Virginia, USA)
