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and international patents and 82 pending U.S
However, highly cationic CPPs can cause cytotoxicity 46 and may escape from vesicles to the cytosol during the transcytosis process due to the electrostatic interaction of the peptide sequence with the negatively charged membrane where they are trapped in, which may facilitate trapping of the peptide in the brain endothelium

Magnetofection PEI-MNPs, T0-CRISPR/Cas9-PEI-MNPs, and T3-CRISPR/Cas9-PEI-MNPs (2 g DNA/well) were incubated with cultured cells in serum free media for 30 min followed by addition of 10% FBS

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Moreover, DHT has been shown to cause hyperinsulinaemia by increasing insulin gene expression in female rats, suggesting that enhanced insulin secretion is due to increased insulin transcription [80]