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In addition, GSH depletion induced SIPS, as evidenced by increased percentage of the senescence-associated -galactosidase-positive cells, increased senescence-associated heterochromatin foci (SAHF), as well as cell cycle arrest at the G1 phase

McGonigle S, Beall MJ, Feeney EL, Pearce EJ

BSO (Sigma-Aldrich, St

Interestingly, GLS1-knockout CD4(+) T cells exhibited an increase in the proportion of T helper 1 (TH1) cells, which showed excessive effector function and an increase in exhaustion signatures, but in cytotoxic CD8+ T cells in culture with limited glutamine or treated with a glutamine metabolism inhibitor prevented exhaustion, promoted memory-like T cell differentiation and tumor elimination in vivo [89, 90]

Glutamate excitotoxicity causes Ca 2+ mediated NO production leading to mitochondrial dysfunction resulting in superoxide production, which is released via voltage dependent anion channels into the cytosol