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Without human trials, every approach is an estimate

In contrast, there is strong genetic and functional evidence that RAS-MAPK activation by canonical agonists or oncogenic RAS mutations is a key collaborator of TGF--activated SMADs in the induction of EMTs (see below)

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Higashi Y, Noma K, Yoshizumi M et al

After filtration and washing, the cells were seeded at 0.25.10 6 cells/well in 24 well plates on 1.3 cm 2 glass coverslips coated with 10 g/mL human fibronectin (Sigma F0556) and cultured for 2 days (about 6070% confluence)