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To enhance the sensitivity in determining ultralow-abundance targets, several effective signal amplification strategies have been integrated with optical immunoassays, including enzyme catalysis, DNA-based amplification, and nanotechnology [12,13,14,15,16]
Subsequently, we successfully established MDA-MB-231 and MDA-MB-468 cells with overexpression of EGR1 (Figure 3A-D)

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These patterns are corroborated by targeted profiling showing negative correlations between alanine, glutamine, threonine, and tyrosine levels and P4 fluctuations [138]

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