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IB, inhibitor of NF-B
2021;7(4) doi: 10.1016/j.heliyon.2021.e06903
The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)
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Additionally, the rapid proliferation of cancer cells leads to increased tumor mass within a confined space, raising intra-tumoral pressure and compressing blood and lymphatic vessels 22
Anthropol Rev 80, 335348
