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2.2.3 Western blot analysis Protein levels of total pyruvate dehydrogenase E1 subunit alpha (PDHA1) and phosphorylated PDHA1 at Ser 293 (pPDHA1) were analyzed in the cytosolic fraction prepared from fresh liver tissue, while representative subunits of mitochondrial oxidative phosphorylation (OXPHOS) complexes IV were determined in whole cell lysates from frozen liver tissue as previously described ( For Western blot analysis, 30 g protein per sample were mixed with loading buffer, denatured at 95 C for 5 min (PDHA1 and pPDHA1) or at 37 C for 20 min (OXPHOS complexes) and separated on a TGX Stain-Free Precast gradient gel (Bio-Rad Laboratories GmbH, Germany)

PMCID: PMC8109558

The treatment itself takes under one hour, often less, and usually a course of 8 sessions is recommended

Q: How long does it take to see the benefits

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The unstable structure of PRIM1 and PRIM2 lead to dysfunction of DNA polymerase- primase complex and stalled replication fork (Liu and Huang, 2015)