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Carbamidomethylation of cysteines was defined as fixed modification, while acetylation of protein N-terminal, oxidation of Methionine was set as variable modifications for database searching

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However, the fluorescence intensity in cells was significantly decreased following the SP treatment, showing a dose-dependent manner (Fig

This included lateral and medial portions of the CC as outlined in histology schematic (Figure 3A)

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The culture medium was removed, and the cells were incubated with serum-free DMEM containing 10 M DCFH-DA at 37 C for 20 min