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Sections were then washed three times before being incubated with secondary antibodies (1:400, species-specific Alexa Flour 488, 555 or 647, Thermo Fisher Scientific), diluted in PBS for 2 h at room temperature on a shaker at 40 rpm

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111,112,113,114 The field of osteochondral tissue engineering has matured substantially and progress towards clinically applicable replacements will be vital for the large portion of the population currently excluded from clinical trials