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These matrix changes may render the degenerated IVD as a more brittle stiffer tissue with less dynamic compliancy properties under load and more prone to the development of fissures in the IVD, further compromising its normal biomechanical functions (Vergroesen et al., 2015)

After each blood sample was taken, the catheter was flushed with heparinized lactated Ringer's solution and a volume of heparinized lactated Ringer's equal to the volume of blood taken was injected (to maintain total blood volume)