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For these studies, virus particles produced in Huh7.5 cells (JFH-1 strain) and secreted into the cell supernatant were highly concentrated by ultracentrifugation through a sucrose cushion, as described in the Materials and Methods section

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The total numbers of hermaphrodite/male intestines examined were L4 (48/17), D1 (37/21), D2 (52/14), D3 (41/22) and D4 (47/16)

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