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The ferroptosis activator RSL-3 and the PI3K/Akt pathway inhibitor LY294002 reversed Neuritins inhibitory effect on ferroptosis, indicating that Neuritin suppresses neuronal ferroptosis via PI3K/Akt pathway activation ( In vitro experiments confirmed that A 25-35 treatment significantly inhibited the activation of the NRF2/SLC7A11/GPX4 signaling pathway in HT22 cells, upregulated the expression of Fe 2+ , TFR1, ACSL4, MDA, and GSSG, and decreased the expression of FTH1, SOD, and GSH, as well as the GSH/GSSG ratio, resulting in dysregulated neuronal iron metabolism, impaired amino acid antioxidant systems, mitochondrial dysfunction, and ferroptosis, which ultimately promoted neuronal injury

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N-acetylcysteine (NAC): Impacts on human health

J Pharmacol Exp Ther 187:211217 Muldrew KL, James LP, Coop L, McCullough SS, Hendrickson HP, Hinson JA, Philip R, Mayeux PR (2002) Determination of acetaminophen-protein adducts in mouse liver and serum and human serum after hepatotoxic doses of acetaminophen using high-performance liquid chromatography with electrochemical detection