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Description
Protein expression For whole cell lysates, cultured cells were rinsed with ice-cold PBS and lysed in RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail
doi: 10.3390/nu13072421 20
Using mass spectrometry, the band was identified as GspD (Table S2), suggesting that secretins are also used by myxobacteria in slime secretion
Biomed Res Int 2019:54037615493770
As one of the virulence factors of Mtb, LAM has the potential to become a vaccine
Passive absorption
