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In the 1990s, researchers discovered that Gila monster saliva contains a peptide called exendin-4, which acts like GLP-1 but resists enzymatic breakdown

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This might explain the lack of pain after PRP injection in most studies, especially the ones using leukocyte poor PRP

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The animals were acclimatised to laboratory conditions (23 C, 12 h/12 h light/dark, 50% humidity, ad libitum access to food and water) for 2 wk prior to experimentation

[DOI] [PMC free article] [PubMed] [Google Scholar] Mohsen K., Azzazy H.M.E., Allam N.K., Basalious E.B