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Molecular modeling with mutagenesis scanning revealed specific regions consisting of hydrophobic residues in ECD (site II
However, the ability of traditional methods to identify regulators of endogenous secreted factors is limited
RistoriMVMorteraSLMarzanoVGuerreraSVernocchiPIaniroGet al
Sperm plasma membrane integrity and acrosome integrity were assessed by randomly selecting three microscopic fields, with each field containing at least 200 spermatozoa
Protein samples were resolved on SDS-PAGE gels and transferred onto a polyvinylidene difluoride (PVDF) membrane via wet transfer
