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Therefore, we anticipated that disrupting the CGATA motif in intron 2 might de-repress c-Kit in erythroid cell lines

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2.8.2 Gene expression analysis of virulence- and cytokines-related genes utilizing reverse transcription-quantitative PCR technique Lung and splenic tissues were collected from 6 rabbits per each experimental group and used to extract total RNA using the QIAamp RNeasy Mini kit (Qiagen, Hilden, Germany) according to the producers manuals

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Because apparent clearance was 3441% lower in the youngest/lightest children, a weight-based (mg/kg) regimen was selected to achieve comparable systemic exposure across all ages and weights and for getting the target (about 4073% across bands) compared to placebo