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It is small, minimally disruptive, and suitable for both native and denaturing conditions

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The example of GSH microspecies B and G (Table 2) shows that even minor changes in the sites of protonation can lead to differences in redox characteristics, this is all the more important in light of the complex, parallel protonation equilibria of these multibasic biomolecules: not only the protonation of the thiolate itself, its protonation fraction, and the concomitant redox behavior will be very sensitive to minor pH changes, but the protonation status of the adjacent basic moieties can influence the redox behavior by various inductive effects on the thiolate moiety

10.1111/ggi.12696 82 GandyS.SimonA

One injection, one time of day, low dose

Metallothionein Zn2+- and Cu2+-clusters from first-principles calculations