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The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

Whitmore et al

Belizario JE, Napolitano M

Oncotarget 8(70):115164115178 Alborzinia H et al (2018) Golgi stress mediates redox imbalance and ferroptosis in human cells

J Immunol 76:417, 1956

This approach holds promise for improving diabetes and its complications ( 3.2.3 Improving diabetes-related cognitive impairment Extensive research has confirmed the significant therapeutic effects of TCM on diabetes-related cognitive dysfunction (DCD)