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[DOI] [PMC free article] [PubMed] [Google Scholar] 36.Snchez-Gmez A., Alcarraz-Vizn G., Fernndez M., Fernndez-Santiago R., Ezquerra M., Cmara A., Serrano M., Novials A., Muoz E., Valldeoriola F., et al

The C-terminal 6xHis-tagged LbcA protein was purified with HiTrap-Ni in 10 mM potassium phosphate, pH 8.0, 0.25 M NaCl, and a 20-CV 10300 mM imidazole gradient, followed by HiTrap-Q in 10 mM Tris, pH 8.0, and a 20-CV 50500 mM NaCl gradient

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