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DOI: 10.1007/s10517-014-2544-7 15
Stage-specific nuclear expression of NF-kappaB in mammalian testis

200 mM sucrose, 5 mM Tris, 1 mM EGTA, 100 g ml 1 digitonin pH 7.4), followed by centrifugation at 1000 g for 1 min at 4 C

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References: [1]afak, Martin

Essentially, the same results were obtained in mice
