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Brain slices were transferred to fresh 4% paraformaldehyde for 48 to 72 h at 4 C with gentle shaking and then transferred to PBS for 24 h at 4 C, followed by dehydration in sucrose gradient (1530%) followed by cryosectioning at 1020 m for immunohistochemistry

Start with bupropion XR 150 mg qAM for three days, then increase to bupropion XR 300 mg qAM