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Briefly, surface TfR or GPI-MFI2 were labeled with anti-TfR and anti-MFI2 antibody at 4 C to block endocytosis in human SK-MEL 28 melanoma cells
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Some treatments are standard (the currently used treatment), and some are being tested in clinical trials

Proc Natl Acad Sci U S A 1997;94:43127.PubMedPMC 60

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a procedure pioneered by Saint Johns Cancer Institute