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3.4 Enzymatic cleavage to remove GST affinity tag Depending on the vector chosen, the GST affinity tag can be removed with thrombin, factor Xa, or PreScission protease, either in solution or while still bound to the column matrix
Atakul T, Altinkaya SO, Abas BI, Yenisey C
SMCs-restricted gene ( Myocd , Tagln , Myh11 , and Cnn1 ) expression maintains SMCs in a differentiated state
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