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doi: 10.1016/j.exger.2018.04.013

The reaction was initiated by adding 60 l of a 50 mM valine solution and incubated at 37 C for 30 min

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LINK Loukas M, Tubbs RS: Comment on Locating the arcuate line of Douglas: Is it of surgical significance? Clin Anat 2010

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