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Exposure of cells (submerged culture) to unheated e-liquid or to one of its specific ingredients is a very inexpensive, simple and high-throughput method for rapid screening

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It is difficult to know exactly what is found in e-cigarettes as there have been some marketed as containing zero percent nicotine when in fact they were tested and did contain nicotine

16 ARE E-CIGARETTES EFFECTIVE FOR SMOKING CESSATION

[41] E-cigarettes have a wide array of engineering designs

The huge variation in study designs assessing in vitro toxicity of e-cigarettes aerosol makes it difficult to make comparisons and draw robust and irrefutable conclusions