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Description
As it makes the person funnier after consumption, wacky was added to make the term sillier but catchy
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Nicotine is metabolized to cotinine primarily by the liver enzyme CYP2A6
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After 2 h of treatment, 10 M of the fluorescence probe 2,7-dichlorofluorescein diacetate (DCFH-DA, D6883, Sigma-Aldrich, MO, USA) was added to the cells
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