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Salmeron, J

Human milk samples should be collected at various time points during the breast-feeding period since the lactational transfer from maternal blood into breast milk can differ, leading to higher levels in colostrum than mature milk

Two-stage culture is another commonly used method for strain screening

After a 1-h incubation at 20 C with agitation worms were washed 4 times in M9 buffer to remove traces of paraquat and spotted on fresh NGM plates with 40 M FUDR and seeded with bacteria

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