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The supernatant was removed and the pellet was washed by adding 800 l pre-cooled (-20 o C) acetone

Crit Rev Oncology/Hematology (2021) 163(103338):103338

10.1523/JNEUROSCI.2737-14.2015 50

There, a protein complex composed of syntaxin1, SNAP25, and synaptobrevin assembles into the SNARE machinery, enabling vesicle docking and priming [191, 192]

2.1.4 Outcome Here, we included the following: neurochemical damage, neurobehavioral impairment, monoamine and metabolite levels, histochemistry, HPLC assay, lipid peroxidase, immunohistochemistry, Western blot assays, RT-PCR, CSF markers, acetylcholinesterase, butyrylcholinesterase, neuroprotective markers, antioxidant levels, oxidative markers, apoptotic markers, histopathology of the brain, immunofluorescence assays, and mitochondrial assays

The CD8 + granzyme B + T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells